Typing and molecular characterization of human papillomaviruses in genital warts from South African women

Abstract

Condyloma acuminata from 12 women were examined for the presence of human papilloma‐virus DNA. Six of the women had HPV 6a, two had HPV 11a, one had a new HPV 6 subtype, and one a new HPV 11 subtype. The new HPV 6 subtype could be distinguished from other HPV 6 subtypes on the basis of Pst 1 fragments as well as Hind II fragments. The new HPV 11 subtype had a different Hpa 11 restriction endonuclease pattern. Restriction maps of both new subtypes were constructed. Two of the biopsies did not contain detectable HPV DNA when probed with HPV 6, HPV 11, and HPV16. Biopsies were taken from normal tissue 1 cm away from the condyloma in 11 of the patients. Only one of these normal tissue biopsies was positive for HPV by Southern blot hybridization.