Synthesis of Types I, III and AB2 Collagen by Chick Tendon Fibroblasts in vitro
- 1 March 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 105 (1), 63-74
- https://doi.org/10.1111/j.1432-1033.1980.tb04474.x
Abstract
Tendons from 14-17 day old chick embryos contain predominantly type I collagen and about 5% AB2 collagen; type III collagen is not detectable by biochemical methods, such as sodium dodecyl sulfate/polyacrylamide gel electrophoresis or CNBr pattern, but can be visualized by immunofluorescence staining with collagen-type-specific antibodies. Freshly dissociated tendon cells secrete only type I collagen into the culture medium but no significant amounts of type III collagen. Transfer of tendon cells from chick embryos to monolayer conditions initiated synthesis of type III collagen in about 10% of the cells within 3 days, as visualized by immunofluorescence staining. Secretion of type III collagen into the culture medium can also be detected by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. With increasing number of passages the number of cells producing type III collagen reached levels of about 80% after the 3rd passage, while 90% of all cells stained positively for type I collagen. This is reflected by an increase of production of type III collagen as determined by CM cellulose chromatography. Using velocity sedimentation, the secretion of type III procollagen and of pN-collagen (carrying the aminoterminal extension only), into the culture medium of a 2nd-passage tendon cell culture was detected. The phenotype of cells may alter during transfer from the environment in vivo to conditions in vitro and additional changes may occur with time in culture.This publication has 37 references indexed in Scilit:
- Fibroblast-like cells from embryonic chick cornea, heart, and skin are antigenically distinctDevelopmental Biology, 1979
- High cell density alters the ratio of type III to I collagen synthesis by fibroblastsNature, 1979
- Isolation and characterization of collagen A and B chains from chick embryosFEBS Letters, 1979
- Characterization of the collagen chains synthesized by cultured smooth muscle cells derived from rhesus monkey thoracic aortaBiochemistry, 1978
- Identification of collagen α1(I) trimer in embryonic chick tendons and calvariaBiochemical and Biophysical Research Communications, 1977
- Immunofluorescent localization of collagen types I, II, and III in the embryonic chick eyeDevelopmental Biology, 1977
- The progeny of rabbit articular chondrocytes synthesize collagen types I and III and type I trimer, but not type II. Verifications by cyanogen bromide peptide analysisBiochemistry, 1977
- Identification of the collagenous proteins synthesized by cultured cells from human skinBiochemistry, 1975
- The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in CultureDifferentiation, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970