Expression of the glyceraldehyde‐3‐phosphate dehydrogenase gene from the extremely thermophilic archaebacterium Methanothermus fervidus in E. coli
- 12 September 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 237 (1-2), 213-217
- https://doi.org/10.1016/0014-5793(88)80204-7
Abstract
The gene of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the extremely thermophilic archaebacterium Methanothermus fervidus (growth optimum 82°C) was cloned in vector pJF118EH and expressed in E. coli cells. As shown by molecular mass determination, protein sequencing, heat stability, and substrate saturation kinetics, the enzyme synthesized in E. coli is identical to the original enzyme from M. fervidus. The high thermostability of the E. coli-produced M. fervidus GAPDH allows rapid purification to homogeneity. From this enzyme protein crystals were grown which proved to be suitable for X-ray analysis. The crystals are of tetragonal space group P4122 and contain a dimer per asymmetric unitKeywords
This publication has 14 references indexed in Scilit:
- Primary structure of glyceraldehyde-3-phosphate dehydrogenase deduced from the nucleotide sequence of the thermophilic archaebacterium Methanothermus fervidusGene, 1988
- Effect of Ribosome Binding Site on Gene Expression inEscherichia coliDNA, 1988
- Thermoadaptation of methanogenic bacteria by intracellular ion concentrationFEMS Microbiology Letters, 1988
- Characterization of two d‐glyceraldehyde‐3‐phosphate dehydrogenases from the extremely thermophilic archaebacterium Thermoproteus tenaxEuropean Journal of Biochemistry, 1987
- Structure and expression of the genes, mcrBDCGA , which encode the subunits of component C of methyl coenzyme M reductase in Methanococcus vannieliiProceedings of the National Academy of Sciences, 1987
- Purification and characterization of d‐glyceraldehyde‐3‐phosphate dehydrogenase from the thermophilic archaebacterium Methanothermus fervidusEuropean Journal of Biochemistry, 1987
- Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vectorGene, 1986
- Determination of the Protein Content of Crystals Formed byMastigocladus laminosusC-Phycocyanin,Chroomonas spec.Phycocyanin-645 and Modified Human Fibrinogen Using an Improved Ficoll Density Gradient MethodBiological Chemistry Hoppe-Seyler, 1985
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977