Single-Channel Recording of a Store-Operated Ca 2+ Channel in Jurkat T Lymphocytes
- 5 February 1999
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 283 (5403), 836-839
- https://doi.org/10.1126/science.283.5403.836
Abstract
In T lymphocytes, a store-operated calcium ion (Ca 2+ ) entry mechanism termed the calcium release–activated Ca 2+ channel (CRAC channel) underlies the sustained or oscillatory intracellular calcium concentration signal required for interleukin-2 gene expression and cell proliferation. The use of sodium ions as a current carrier enabled single-channel recordings of CRAC channels during activation, inactivation, and blockade of current in the presence of divalent cations. A large conductance of 36 to 40 picosiemens indicates that 100 to 400 CRAC channels are present in T lymphocytes.Keywords
This publication has 14 references indexed in Scilit:
- Conductance and permeation of monovalent cations through depletion-activated Ca2+ channels (ICRAC) in Jurkat T cellsBiophysical Journal, 1996
- Potassium and Calcium Channels in LymphocytesAnnual Review of Immunology, 1995
- Mitogen-regulated Ca2+ current of T lymphocytes is activated by depletion of intracellular Ca2+ stores.Proceedings of the National Academy of Sciences, 1993
- Calcium release‐activated calcium current in rat mast cells.The Journal of Physiology, 1993
- Depletion of intracellular calcium stores activates a calcium current in mast cellsNature, 1992
- Capacitative calcium entry revisitedCell Calcium, 1990
- Mitogen-induced oscillations of cytosolic Ca2+ and transmembrane Ca2+ current in human leukemic T cells.Cell Regulation, 1989
- Calcium channel selectivity for divalent and monovalent cations. Voltage and concentration dependence of single channel current in ventricular heart cells.The Journal of general physiology, 1986
- The Ca channel in skeletal muscle is a large pore.Proceedings of the National Academy of Sciences, 1985
- Mechanism of ion permeation through calcium channelsNature, 1984