Molecular cloning of rat kynurenine aminotransferase: Identity with glutamine transaminase K
Open Access
- 10 October 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 353 (1), 21-24
- https://doi.org/10.1016/0014-5793(94)01003-x
Abstract
The enzyme kynurenine aminotransferase (KAT) catalyses the conversion of l‐kynurenine to kynurenic acid. A combination of polymerase chain reaction techniques and hybridization screening was used to isolate a cDNA clone encompassing the entire coding region of KAT from rat kidney. Identification of the cDNA as coding for KAT was based both on the comparison of amino acid sequences obtained from purified rat KAT and on the expression of KAT activity in COS‐1 cells transfected with the cDNA. RNA blot analysis indicated that KAT mRNA is widely expressed in rat tissues. Cultured cells transfected with the cDNA for KAT also showed glutamine transaminase K activity. Based mainly on sequence data, these results demonstrate that rat kidney KAT is identical with glutamine transaminase K.Keywords
This publication has 14 references indexed in Scilit:
- Renal endothelin gene expression is increased in remnant kidney and correlates with disease progressionKidney International, 1993
- Kynurenic Acid: A Potential Pathogen in Brain DisordersaAnnals of the New York Academy of Sciences, 1992
- Measurement of Rat Brain Kynurenine Aminotransferase at Physiological Kynurenine ConcentrationsJournal of Neurochemistry, 1991
- The signal peptide of human preproendothelin‐1FEBS Letters, 1991
- Purification and characterization of kynurenine-pyruvate aminotransferase from rat kidney and brainBrain Research, 1990
- Nucleotide sequence of cDNA coding for saporin‐6, a type‐1 ribosome‐inactivating protein from Saponaria officinalisEuropean Journal of Biochemistry, 1989
- Kynurenate inhibition of cell excitation decreases stroke size and deficitsAnnals of Neurology, 1987
- Inhibition of excitatory neurotransmission with kynurenate reduces brain edema in neonatal anoxiaNeuroscience Letters, 1986
- Kynurenic acid blocks neurotoxicity and seizures induced in rats by the related brain metabolite quinolinic acidNeuroscience Letters, 1984
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977