EMBRYO VACCINATION AGAINST EIMERIA TENELLA AND E. ACERVULINA INFECTIONS USING RECOMBINANT PROTEINS AND CYTOKINE ADJUVANTS
- 1 June 2005
- journal article
- Published by American Society of Parasitologists in Journal of Parasitology
- Vol. 91 (3), 666-673
- https://doi.org/10.1645/ge-3476
Abstract
Avian coccidiosis is an intestinal disease caused by protozoa of the genus Eimeria. To investigate the potential of recombinant protein vaccines to control coccidiosis, we cloned 2 Eimeria sp. genes (EtMIC2 and 3-1E), expressed and purified their encoded proteins, and determined the efficacy of in ovo immunization to protect against Eimeria infections. Immunogen-specific serum antibody titers, parasite fecal shedding, and body weight gains were measured as parameters of disease. When administered alone, the recombinant EtMIC2 gene product induced significantly higher antibody responses, lower oocyst fecal shedding, and increased weight gains compared with nonvaccinated controls following infection with E. tenella. Combined embryo immunization with the EtMIC2 protein plus chicken cytokine or chemokine genes demonstrated that all 3 parameters of vaccination were improved compared with those of EtMIC2 alone. In particular, covaccination with EtMIC2 plus interleukin (IL)-8, IL-16, transforming growth factor-β4, or lymphotactin significantly decreased oocyst shedding and improved weight gains beyond those achieved by EtMIC2 alone. Finally, individual vaccination with either EtMIC2 or 3-1E stimulated protection against infection by the heterologous parasite E. acervulina. Taken together, these results indicate that in ovo vaccination with the EtMIC2 protein plus cytokine/chemokine genes may be an effective method to control coccidiosis.Keywords
This publication has 29 references indexed in Scilit:
- Intestinal Immunomodulation by Vitamin A Deficiency and Lactobacillus-Based Probiotic in Eimeria acervulina–Infected Broiler ChickensAvian Diseases, 2003
- Isolation and Characterization of Chicken Interleukin-17 cDNAJournal of Interferon & Cytokine Research, 2002
- Adjuvant effects of IL-1β, IL-2, IL-8, IL-15, IFN-α, IFN-γ TGF-β4 and lymphotactin on DNA vaccination against Eimeria acervulinaVaccine, 2001
- cDNA Cloning of Biologically Active Chicken Interleukin-18Journal of Interferon & Cytokine Research, 2000
- A Recombinant Eimeria Protein Inducing Interferon-g Production: Comparison of Different Gene Expression Systems and Immunization Strategies for Vaccination against CoccidiosisAvian Diseases, 2000
- Embryo vaccination of turkeys against Newcastle disease infection with recombinant fowlpox virus constructs containing interferons as adjuvantsVaccine, 1999
- In Ovo Vaccination Technology *The authors are employees and consultants of Embrex, Inc., which disclaims responsibility for any private publication of its personnel. The views expressed herein do not necessarily reflect the views of Embrex, Inc. or its management.Published by Elsevier BV ,1999
- Applications in in ovo technologyPoultry Science, 1997
- Molecular cloning and characterization of a novel acidic microneme protein (Etmic-2) from the apicomplexan protozoan parasite, Eimeria tenellaMolecular and Biochemical Parasitology, 1996
- CYTOKINES: COORDINATORS OF IMMUNE AND INFLAMMATORY RESPONSESAnnual Review of Biochemistry, 1990