cDNA Cloning of Biologically Active Chicken Interleukin-18
- 1 October 2000
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Interferon & Cytokine Research
- Vol. 20 (10), 879-883
- https://doi.org/10.1089/10799900050163244
Abstract
By searching a chicken EST database, we identified a cDNA clone that appeared to contain the entire open reading frame (ORF) of chicken interleukin-18 (ChIL-18). The encoded protein consists of 198 amino acids and exhibits approximately 30% sequence identity to IL-18 of humans and various others mammals. Sequence comparisons reveals a putative caspase-1 cleavage site at aspartic acid 29 of the primary translation product, indicating that mature ChIL-18 might consist of 169 amino acids. Bacterially expressed ChIL-18 in which the N-terminal 29 amino acids of the putative precursor molecule were replaced by a histidine tag induced the synthesis of interferon-γ (IFN-γ) in cultured primary chicken spleen cells, indicating that the recombinant protein is biologically active.Keywords
This publication has 32 references indexed in Scilit:
- Interleukin-18 (IFNgamma-inducing factor) induces IL-8 and IL-1beta via TNFalpha production from non-CD14+ human blood mononuclear cells.Journal of Clinical Investigation, 1998
- IGIF Does Not Drive Th1 Development but Synergizes with IL-12 for Interferon-γ Production and Activates IRAK and NFκBImmunity, 1997
- Activation of Interferon-γ Inducing Factor Mediated by Interleukin-1β Converting EnzymeScience, 1997
- Biological properties of recombinant chicken interferon‐γEuropean Journal of Immunology, 1996
- A Family of Genes Coding for Two Serologically Distinct Chicken InterferonsJournal of Biological Chemistry, 1996
- A newly defined interleukin-1?Nature, 1996
- Cloning and Expression of the Chicken Interferon-γ GeneJournal of Interferon & Cytokine Research, 1995
- Cloning of a new cytokine that induces IFN-γ production by T cellsNature, 1995
- Nitric oxide. A macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells.The Journal of Experimental Medicine, 1989
- Chicken hematopoietic cells transformed by seven strains of defective avian leukemia viruses display three distinct phenotypes of differentiationCell, 1979