Interaction and Comparison of a Class I Hydrophobin from Schizophyllum commune and Class II Hydrophobins from Trichoderma reesei
- 18 March 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 7 (4), 1295-1301
- https://doi.org/10.1021/bm050676s
Abstract
Hydrophobins fulfill a wide spectrum of functions in fungal growth and development. These proteins self-assemble at hydrophilic−hydrophobic interfaces into amphipathic membranes. Hydrophobins are divided into two classes based on their hydropathy patterns and solubility. We show here that the properties of the class II hydrophobins HFBI and HFBII of Trichoderma reesei differ from those of the class I hydrophobin SC3 of Schizophyllum commune. In contrast to SC3, self-assembly of HFBI and HFBII at the water−air interface was neither accompanied by a change in secondary structure nor by a change in ultrastructure. Moreover, maximal lowering of the water surface tension was obtained instantly or took several minutes in the case of HFBII and HFBI, respectively. In contrast, it took several hours in the case of SC3. Oil emulsions prepared with HFBI and SC3 were more stable than those of HFBII, and HFBI and SC3 also interacted more strongly with the hydrophobic Teflon surface making it wettable. Yet, the HFBI coating did not resist treatment with hot detergent, while that of SC3 remained unaffected. Interaction of all the hydrophobins with Teflon was accompanied with a change in the circular dichroism spectra, indicating the formation of an α-helical structure. HFBI and HFBII did not affect self-assembly of the class I hydrophobin SC3 of S. commune and vice versa. However, precipitation of SC3 was reduced by the class II hydrophobins, indicating interaction between the assemblies of both classes of hydrophobins.Keywords
This publication has 55 references indexed in Scilit:
- TheTrichoderma reeseihydrophobin geneshfb1andhfb2have diverse functions in fungal developmentFEMS Microbiology Letters, 2005
- Hydrophobins: the protein-amphiphiles of filamentous fungiFEMS Microbiology Reviews, 2005
- MHP1, a Magnaporthe grisea hydrophobin gene, is required for fungal development and plant colonizationMolecular Microbiology, 2005
- Hydrophobins: proteins with potentialCurrent Opinion in Biotechnology, 2005
- Surface adhesion of fusion proteins containing the hydrophobins HFBI and HFBII from Trichoderma reeseiProtein Science, 2002
- Structural changes and molecular interactions of hydrophobin SC3 in solution and on a hydrophobic surfaceProtein Science, 2002
- Self‐assembly of the hydrophobin SC3 proceeds via two structural intermediatesProtein Science, 2002
- Spectroscopic Evidence for Amyloid-like Interfacial Self-Assembly of Hydrophobin Sc3Biochemical and Biophysical Research Communications, 2001
- Hydrophobins line air channels in fruiting bodies of Schizophyllum commune and Agaricus bisporusMycological Research, 1999
- The thn mutation of Schizophyllum commune, which suppresses formation of aerial hyphae, affects expression of the Sc3 hydrophobin geneJournal of General Microbiology, 1991