Acetylcholinesterase-containing nerve fibers in the dura mater of guinea pig, mouse, and rat

Abstract
The cholinergic innervation of the dura mater in the guinea pig, mouse and rat has been investigated. The dura mater is provided with a cholinergic innervation. After short incubation times (4–6 hours) we found acetylcholinesterase (AChE)-containing nerve fibers in close relationship with the main meningeal blood vessels. After longer incubation times, AChE-containing nerve fibers appear also in the meningeal tissue proper. Chemical sympathectomy, performed with the neurotoxin 6-hydroxydopamine, does not seem to interfere with the pattern of the dural cholinergic innervation. The findings are discussed.