Plasminogen activator induction and platelet aggregation by phorbol and some of its derivatives: Correlation with skin irritancy and tumor-promoting activity
- 1 August 1980
- journal article
- research article
- Published by Springer Nature in Zeitschrift für Krebsforschung und Klinische Onkologie
- Vol. 97 (3), 257-266
- https://doi.org/10.1007/bf00405777
Abstract
Phorbol und acht seiner Derivate wurden auf ihre Fähigkeit untersucht, die Synthese von Plasminogen-Aktivator in Zellkulturen von Hühnerembryo-Fibroblasten zu stimulieren und die Aggregation von Blutplättchen zu induzieren und auf ihre tumorpromovierende und hautirritierende Wirkung getestet. Die Erhöhung der Plasminogen-Aktivator Aktivität durch Phorbolderivate korreliert gut mit ihren irritierenden und promovierenden Eigenschaften. Im Test auf Blutplättchen-Aggregation ist die Korrelation nicht eindeutig: Sie gilt für starke Promotoren (wie TPA), die auch hochwirksame Induktoren der Aggregation sind, sowie für schwache Promotoren (wie PDA), die nur gering oder nicht induzieren; Ausnahmen sind PDD und PDB: PDD, ein starker Promoter, ist nur schwach wirksam, PDB, ein schwacher Promotor, ist dagegen das am stärksten aggregationsstimulierende Derivat. Phorbol and eight of its derivatives were investigated for their ability to stimulate the synthesis of the enzyme plasminogen activator in cultured chick embryo fibroblasts and to aggregate human blood platelets and have been assayed for tumor, promoting and skin, irritant activities. Over a range of concentrations, elevation in the levels of plasminogen activator activity induced by phorbol derivatives correlates well with their promoting and irritant properties. In the platelet aggregation assay however, the parallelism between the activities measured in different biological assays was less complete. While strong promoters, such as TPA, are potent aggregating agents, and weak promoters, such as PDA, are poor or ineffective inducers of aggregation, two derivatives, PDD and PDB, deviate from this general result. Platelets must be exposed to PDD in relatively high concentrations before they will aggregate, and PDB was found to be the most potent aggregating agent of all the derivatives tested.Keywords
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