Properties of Magnesium Chelatase in Greening Etioplasts

Abstract
Evidence was obtained by low temperature spectrofluorimetry and high pressure liquid chromatography for the exclusive chelation of Mg2+ into protoporphyrin IX by a plastid preparation extracted from greening cucumber [Cucumis sativa cv. Beit Alpha] cotyledons. Under experimental conditions which were optimized for Mg2+ chelation Zn2+ chelation was not detected. Zn2+ chelation was observed when ATP was omitted and ZnCl2 was added to the incubation mixture. The observed Zn2+ chelation was partially nonenzymic. The requirements for the enzyme, Mg chelatase, are discussed for its regulatory function.