CYCLIC AMP AND CYCLIC GMP ACCUMULATION BY ISOLATED HAMSTER GRANULOSA CELLS STIMULATED BY LH AND FSH

Abstract

Cyclic[c]AMP and cGMP accumulation in hamster granulosa cells as a function of gonadotropin dose (LH [lutropin] or FSH [Follitropin]) and time (0-30 min) was determined. The pattern of acute cAMP accumulation was similar in LH and FSH stimulated granulosa cells, except that the cells were more sensitive to FSH and LH. There was a positive dose response relationship of cAMP accumulation in LH and FSH stimulated cells. LH appeared to partially inhibit FSH stimulated cAMP synthesis. cGMP accumulation was distinctly different in LH and FSH stimulated cells. An inverse dose response relationship of cGMP to dose LH was observed, with only the lowest dose of LH (0.005 IU/ml) stimulating cGMP synthesis. FSH at 0.005 IU/ml did not stimulate cGMP synthesis, but at higher doses generated cGMP in a positive dose-related manner. Specificity of hormone action in granulosa cells may be governed in part by differentiation between cAMP and cGMP in these cells.