An enzyme electrode for specific determination of L‐lysine: A real‐time control sensor
- 18 February 1983
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 25 (2), 329-340
- https://doi.org/10.1002/bit.260250203
Abstract
Lysine decarboxylase (L‐lysine carboxylyase, E.C.4.1.1.18) is immobolized on a carbon dioxide gas‐sensing electrode, by copolymerization with gelatin using the bifuncitional agent glutaraldehyde. The enzyme electrodes thus prepared are used in a continuous flow system to measure the concentration of L‐lysine in a mixture of amino acids. The measuring time for each sample is about 3 min, including response and rinsing times. The electrode response is linear between 0.01‐1 g/L and has a high specificity for L‐lysine. The enzyme electrode response to lysine at concentrations below 0.5 g/L is stable on repeated use for at least 500 assays.This publication has 15 references indexed in Scilit:
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