Effects of Naloxone on Canine Cerebral Vascular Smooth Muscle

Abstract

The pharmacological effects of naloxone on cerebral arterial smooth muscle in vitro were examined using canine basilar arterial strips. Naloxone exerted two different effects on canine basilar artery: (1) at a high concentration (3 × 10⁻⁴ M) it produced nonspecific vasodilation, and (2) at lower concentrations (3 × 10⁻⁷, 3 × 10⁻⁶, and 3 × 10⁻⁵ M) it inhibited the vasoconstrictor effects of norepinephrine without altering KCl-, serotonin-, or hemoglobin-induced constriction. Morphine (2 × 10⁻⁵ or 2 × 10⁻⁴ M) did not reverse the specific vasodilating effect of naloxone (3 × 10⁻⁵ M) on norepinephrine-induced constriction. Rather, morphine and naloxone together produced a greater vasodilating effect on norepinephrine-induced constriction than either agent alone. Naloxone (3 × 10⁻⁵ M) failed to alter either phenylephrine-induced constriction or clonidine-induced constriction. The vasodilating effect of naloxone (3 × 10⁻⁵ M) on 10⁻³ M norepinephrine-induced constriction was not reduced with 10⁻⁶ M propranolol. These results suggest that the vasodilating effect of naloxone on norepinephrine-induced constriction does not result from an antagonistic action on opiate receptors, direct inhibition of α-adrenoreceptors, or direct stimulation of β-adrenoreceptors in canine cerebral arterial smooth muscle. The vasodilating effect of naloxone on norepinephrine-induced constriction may influence the CBF changes following naloxone administration.