Subcellular localization of histone messenger RNAs on cytoskeleton‐associated free polysomes in HeLa S3 cells
- 31 October 1985
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 125 (2), 345-353
- https://doi.org/10.1002/jcp.1041250225
Abstract
We have examined the subcellular distribution of histone mRNA-containing polysomes in HeLa S3 cells to assess the possible relationship between localization of histone mRNAs and the regulation of cellular histone mRNA levels. The distribution of histone mRNAs on free and membrane bound polysomes was examined as well as the association of histone mRNA-containing polysomes with the cytoskeleton. The subcellular localization of histone mRNAs was compared with that of HLA-B7 mRNAs which encode a cell surface antigen. Histone mRNAs were localized predominantly on the free polysomes, whereas the HLA-B7 mRNA was found almost exclusively on membrane bound polysomes. However, both species of mRNA were found associated with the cytoskeleton. Interruption of DNA synthesis by hydroxyurea treatment resulted in a rapid and selective destabilization of histone mRNAs in each subcellular fraction; in contrast, the stability of HLA-B7 mRNA appeared unaffected. The results presented confirm that histone mRNAs are predominantly located on non-membrane bound polysomes and suggest that these polysomes are associated with the cytoskeletal framework.This publication has 51 references indexed in Scilit:
- Inhibition of DNA replication coordinately reduces cellular levels of core and H1 histone mRNAs: requirement for protein synthesisBiochemistry, 1984
- Expression of Vaccinia Virus Early mRNA in Ehrlich Ascites Tumor CellsEuropean Journal of Biochemistry, 1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- The cytoskeletal framework and poliovirus metabolismCell, 1979
- Hybridization Analysis of Histone Messenger RNA: Association with Polyribosomes During the Cell CycleScience, 1975
- On the Translational Control of Histone SynthesisEuropean Journal of Biochemistry, 1974
- Synthesis of histones in a rabbit reticulocyte cell-free system directed by a polyribosomal RNA fraction from synchronized Hela cellsBiochemical and Biophysical Research Communications, 1972
- THE SYNTHESIS OF ACIDIC CHROMOSOMAL PROTEINS DURING THE CELL CYCLE OF HELA S-3 CELLSThe Journal of cell biology, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- THE SYNTHESES OF TOTAL MACRONUCLEAR PROTEIN, HISTONE, AND DNA DURING THE CELL CYCLE IN EUPLOTES EURYSTOMUS The Journal of cell biology, 1966