Quantitation of Glycosylated Hemoglobin: Elimination of Labile Glycohemoglobin During Sample Hemolysis at pH 5

Abstract

A simple method for the elimination of labile glycohemoglobin in the chromatographic quantitation of glycosylated hemoglobin is described. Use is made of the instability of Schiff base adducts in acidic solution. Erythrocytes are lysed with a pH 5 buffer. At this pH dissociation reaches completion during sample preparation.