Micronucleus Induction in Human B and T Lymphocytes Separated by an Immunomagnetic Technique
- 28 May 2004
- journal article
- Published by Springer Nature in Hereditas
- Vol. 119 (2), 99-103
- https://doi.org/10.1111/j.1601-5223.1993.00099.x
Abstract
Micronuclei were induced in human lymphocytes by mitomycin C or gamma radiation. After the incubation the cells were separated in B- and T-cells by an immunomagnetic method, i.e., Dynabeads. The cultures were stimulated by PHA. The effect of different incubation times (60-110 h) as well as different doses of mitomycin C (0-100 nmol/l) and gamma radiation (0-2 Gy) at an incubation time of 72 h were studied. There were significantly higher frequencies of micronuclei in B- than in T-cells, when micronuclei were induced by mitomycin C. This was not found with gamma radiation, probably because of a cytotoxic effect of this agent on the B-cells.Keywords
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