Fermentation, isolation and characterization of antibiotic PR-1350.

Abstract

A number of strains of Oidiodendron truncatum produced a new antibiotic, PR-1350, which was isolated in the form of an amorphous powder directly or via a crystalline monomethanolate, PR-1381, which in solution is reconverted to the parent compound. The antibiotic inhibited a broad spectrum of gram-positive and gram-negative bacteria in vitro and was active against P-388 lymphocytic leukemia in mice. Biosynthetic considerations based on the results of [1-13C]acetate incorporation indicated that the antibiotic is a diterpene of the clerodane type.