Nonrandom integration of human U4 RNA pseudogenes.
Open Access
- 1 May 1985
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 5 (5), 943-948
- https://doi.org/10.1128/mcb.5.5.943
Abstract
Four loci for human U4 RNA have been characterized by DNA sequence analysis. The results show that all four loci represent pseudogenes, which are flanked by direct repeats. Three of the pseudogenes, designated U4/5, U4/6, and U4/8, have very similar structures; they are all truncated and contain the first 67 to 68 nucleotides of the U4 RNA sequence. Their properties suggest that they were created by integration of truncated cDNA copies of the U4 RNA into new chromosomal sites. An interesting observation was that their flanking regions exhibit sequence homology. A purine-rich 5'-flanking sequence 12 to 13 nucleotides long is almost perfectly conserved in all three loci. Boxes of homology were also found on the 3' side when the U4/6 and U4/8 loci were compared. The U4/4 locus has a slightly different structure; the pseudogene matches the first 79 nucleotides of U4 RNA, but contains a greater number of mutations than the other pseudogenes. Taken together, the results suggest that a frequently occurring type of pseudogene for human U4 was created by a RNA-mediated mechanism and that the integration sites have features in common.This publication has 45 references indexed in Scilit:
- Genes and pseudogenes for human U2 RNAJournal of Molecular Biology, 1984
- Are U4 small nuclear ribonucleoproteins involved in polyadenylation?Nature, 1984
- Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoproteinCell, 1983
- Demonstration of free reverse transcriptase in the nuclei of embryonic tissues of the Japanese quailBiochemical and Biophysical Research Communications, 1983
- Loci for human U1 RNA: Structural and evolutionary implicationsJournal of Molecular Biology, 1983
- Pseudogenes for human small nuclear RNA U3 appear to arise by integration of self-primed reverse transcripts of the RNA into new chromosomal sitesCell, 1983
- Transcription of a gene for human U1 small nuclear RNACell, 1982
- The isolation and characterization of linked δ- and β-globin genes from a cloned library of human DNACell, 1978
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975