Analysis of variance study of the rat cortical layer 4 barrel and layer 5b neurones

Abstract

Unique formation of rodent cortical barrels by layer 4 neurones attracts study of the sensory function of cortical input stage neurones (layer 4) compared with that of output stage neurones (layer 5). We have recorded extracellular responses from rat somatosensory cortical neurones to deflections of contralateral vibrissae. Thirty‐two layer 4 barrel neurones and 29 layer 5b neurones were studied. Whisker stimulations were ramp‐and‐hold deflections with one of six different ramp velocities (100–2.5 mm s⁻¹) and one of four different plateau amplitudes (2000–200 μm). Twenty‐four (6 × 4) different stimulus forms were applied to the tip of a whisker trimmed to 10 mm in a predetermined order in stimulus cycles of 20–50 repetitions. Spike counts for a period of 2560 ms in 10 ms bins were summed to construct a matrix of 24 peristimulus histograms for each neurone. Twenty‐four amplitude and 24 velocity values were computed from counts during the plateau and ramp phases, respectively. To determine the amplitude‐ and velocity dependence of a neurone, an amplitude F value (the ratio of variations among‐/within‐amplitude of the amplitude value) and a velocity F value (ratio of variations among‐/within‐velocity of the velocity value) were derived by analysis of variance. The amplitude F value of the layer 4 barrel neurones was greater than that of the layer 5b neurones (P < 0.0001). The velocity F value of the barrel neurones was smaller than that of the layer 5b neurones (P = 0.0226). The results suggests that barrel neurones and layer 5b neurones tend to detect amplitude and velocity components of whisker deflection, respectively.