The role of Na+ and Ca+ions on the action of pancreatic lipase studied with the help of immobilisation techniques

Abstract

The role of Na+ and Ca2+ as well as the influence of other metal ions on the [pig intestine] lipase action were studied with the aid of immobilization techniques. By this method it is possible to distinguish between the action of these ions on the lipase molecule itself and their action on the substrate or product. Both alkali and alkali earth metal ions, especially Na+, Ca2+ and Mg2+, stabilize active states of the enzyme which were detected by immobilization to controlled pore glass beads. Though Na+ as well as Ca2+ and Mg2+ stabilize the enzyme significantly, they differ in their efficacy. Reasons for this are discussed and the data compared to the findings of other authors who performed their studies with the native enzyme.