Anin vitroRNA polymerase III system fromS. cerevisiae:effects of deletions and point mutations uponSUP4gene transcription
Open Access
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (24), 8127-8143
- https://doi.org/10.1093/nar/10.24.8127
Abstract
A soluble cell–free extract containing RNA polymerase III – and factors essential for selective transcription of the yeast SUP4–tRNAtyr gene was prepared from Saccharomyces cerevisiae cells. An intragenic promoter for yeast RNA Polymerase III was identified within the yeast tRNAtyr coding sequence by testing several SUP4 genes with 5′– and 3′–terminal deletions in the homologous transcription system.Keywords
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