An abundant ubiquitous glycoprotein (GP100) in nucleated mammalian cells

Abstract

Two‐dimensional gel electrophoresis with the ¹²⁵I‐Con A overlay and affinity purification with Con A‐agarose revealed the presence of an abundant ubiquitous 100‐kDa glycoprotein (GP₁₀₀) in nucleated mammalin cells. The amount in cultured human and murine cells varies from 3 to 20 × 10⁶ molecules per cell making GP₁₀₀ the most abundant glycoprotein in nucleated cells. Peptide mapping shows that it is different from erythrocyte Band III protein. Several properties of GP₁₀₀ suggest that it could play a structural role in nucleated cell membranes.