Kernmagnetische-Resonanz-Messungen an Aminoacyl-adenosin aus Aminoacyl-s-RNA und an Modellsubstanzen

Abstract

All the signals in the nuclear magnetic resonance spectra of aminoacyl adeno-sines were identified by comparison with the spectra of model compounds. According to the NMR [nuclear magnetic resonance] spectra, amino acyl adenosine isolated from aminoacyl s-RNA consisted of more than 90% of the 3[image]-ester, whereas synthetically prepared valyl, alanyl, and methionyl adenosines contained about 70% of tne 3[image]- and 30% of the 2[image]-ester. From model experiments with the synthetic aminoacyl adenosines it seems unlikely that the high amount of 3[image]-ester in aminoacyl adenosine from amino-acyl s-RNA is formed during the isolation process.