The isozyme pattern of glutathione S‐transferases in rat heart

Abstract
The isozyme pattern of glutathione S‐transferases of rat heart differs markedly from that of liver. Heart lacks the majority of basic isozymes recognized in rat liver, and isoelectric focusing of glutathione S‐transferases obtained from affinity chromatography on S‐hexylglutathione‐linked Sepharose 6B revealed that 3 major acidic isozymes with pI values of 6.8, 6.3 and 4.9 are present in heart, at least two of which were not found in liver. In addition, multiple bands were found in the pI range 5.0–5.5 for glutathione S‐transferase activity and protein staining.