Proteome analysis of rat pancreatic acinar cells: Implication for cerulein‐induced acute pancreatitis
- 8 December 2003
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 3 (12), 2446-2453
- https://doi.org/10.1002/pmic.200300545
Abstract
Cerulein pancreatitis was shown to be one of the best characterized models for acute pancreatitis. High doses of cerulein induce a dysregulation of the digestive enzyme production and cytoplasmic vacuolization and the death of acinar cells, edema formation, and an infiltration of inflammatory cells into the pancreas, which are similar symptoms shown in human acute pancreatitis. The present study aims to determine the differentially expressed proteins in cerulein‐treated pancreatic acinar cells as an in vitro model for acute pancreatitis. Pancreatic acinar AR42J cells were treated with 10−8 M cerulein for 24 h. The changed protein patterns separated by two‐dimensional electrophoresis using pH gradients of 5–8 were conclusively identified by matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry analysis of the peptide digests. Five differentially expressed proteins (heat shock protein 90, mitochondrial ATP synthase beta chain precursor, tubulin beta chain, 3‐mercaptopyruvate sulfurtransferase, mitochondrial ATP synthase subunit D) were identified in cerulein‐treated AR42J cells. These proteins are related to cellular stress such as reactive oxygen species, cytoskeletal function, and cell signaling. In conclusion, the differentially expressed proteins will provide valuable information to understand the pathophysiologic mechanism of acute pancreatitis and may be useful for prognostic indices of acute pancreatitis.Keywords
This publication has 32 references indexed in Scilit:
- Geldanamycin inhibits the production of inflammatory cytokines in activated macrophages by reducing the stability and translation of cytokine transcriptsArthritis & Rheumatism, 2003
- Dose-dependent inhibition of mitochondrial ATP synthase by 17β-estradiolGynecological Endocrinology, 2002
- Induction of heat shock proteins fails to produce protection against trypsin-induced acute pancreatitis in ratsClinical and Experimental Medicine, 2002
- Comparative effects of water immersion pretreatment on three different acute pancreatitis models in ratsBiochemistry and Cell Biology, 2002
- Tissue and intracellular distribution of rhodanese and mercaptopyruvate sulphurtransferase in ruminants and birdsVeterinary Research, 2001
- Water immersion pretreatment decreases pro-inflammatory cytokine production in cholecystokinin-octapeptide-induced acute pancreatitis in rats: possible role of HSP72International Journal of Hyperthermia, 2001
- Role of NF-κB in immune and inflammatory responses in the gutGut, 1998
- Mechanics of motility: distinct dynein binding domains on α- and β-tubulinBiochemistry and Cell Biology, 1995
- Effects of Secretin and Caerulein on Pancreatic Digestive Enzymes in Cultured Rat Acinar CellsPancreas, 1994
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976