Increase of serum very low density lipoproteins in rats after administration of ?-hexachlorocyclohexane

Abstract

After enteral administration of 200 mg/kg α-hexachlorocyclohexane (α-HCH) female Wistar rats develop a hyperlipemia. 48 h after administration of α-HCH, serum triglycerides are increased by 300%, whereas both serum cholesterol and serum total phospholipids only increase by about 45%. Serum free fatty acids are not significantly altered. Fractionation of the serum lipoproteins by ultracentrifugation shows that the hyperlipemia is due to a fivefold increase in serum very low density lipoproteins. Hepatic triglyceride secretion, calculated after i.v. injection of Triton WR 1339, is increased in animals pretreated with α-HCH. Corresponding to this observation, drugs known to diminish the triglyceride secretion of the liver, such as actinomycin D, cycloheximide; glucagon, orotic acid, CFT 1201, and CFT 1042 reduce the α-HCH-induced hyperlipemia. We concluded from the results that hyperlipoproteinemia after α-HCH is due to an increased hepatic very low density lipoprotein secretion. At the same time, the blood sugar level was decreased in fasting animals after treatment with α-HCH. Earlier experiments suggest that this effect is due to a decreased gluconeogenesis in the liver.