Cell wall invertases from apex and callus tissues of sugar cane

Abstract

Calluses were obtained from the stalk apex of sugar cane. Both the stalk apex and callus tissues possessed firmly bound cell wall invertases. The invertases of each tissue were characterized on the basis of their Km, optimum pH and the action of various inhibitors. According to this characterization, the two tissues possess different isoenzymes. Taking into account the present isoenzymes and the known cell wall invertases from stalk tissue, we postulated a different pattern of isoenzymes for each organ of the sugar cane. These differences suggest that the cell wall invertases might be used as markers in studies of tissue differentiation.