Three-dimensional reconstruction of a peroxisomal reticulum in regenerating rat liver: evidence of interconnections between heterogeneous segments.
Open Access
- 1 August 1987
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 105 (2), 713-722
- https://doi.org/10.1083/jcb.105.2.713
Abstract
The three-dimensional (3-D) form and the interrelationship of peroxisomes (Po) in the model of regenerating rat liver after partial hepatectomy were studied by computer-assisted 3-D reconstruction of serial ultrathin sections. Po were labeled cytochemically for either catalase, which stains them all uniformly, or for D-amino acid oxidase (DAA-OX), which gives a heterogeneous reaction with lightly and darkly stained PO. In regenerating rat liver, Po exhibit marked pleomorphism with some budding forms and dumbbell-shaped ones. The 3-D reconstruction revealed many single spherical Po measuring 0.15-0.8 micron in diameter. In addition, two to five Po were found interconnected with each other via narrow 30-50-nm hourglass-shaped bridges forming a reticulum. Such aggregates of Po measured 1.5-2.5 microns across. Whereas all segments of this reticulum stained homogeneously for catalase, they exhibited a marked difference in the intensity of the DAA-OX reaction. These observations are consistent with the view of peroxisomal proliferation by budding or fragmentation from preexisting ones. Under such conditions of rapid growth as in regenerating liver, Po may be interconnected forming a reticulum. The interconnections between Po with differing DAA-OX activities suggest that they originate from the same parent organelle.Keywords
This publication has 44 references indexed in Scilit:
- TWO TYPES OF MICROBODIES IN NEUROSPORA CRASSA*Annals of the New York Academy of Sciences, 1982
- Selective cytochemical localization of peroxidase, cytochrome oxidase and catalase in rat liver with 3,3?-diaminobenzidineHistochemistry and Cell Biology, 1981
- The activities of peroxisomal oxidases in periportal and perivenous zones of the rat liver acinusHistochemistry and Cell Biology, 1980
- Cytochemical localization of catalase and several hydrogen peroxide-producing oxidases in the nucleoids and matrix of rat liver peroxisomesJournal of Molecular Histology, 1979
- Microbodies in experimentally altered cells. 8. Continuities between microbodies and their possible biologic significance.1971
- MICROBODY FORMATION IN REGENERATING RAT LIVERJournal of Histochemistry & Cytochemistry, 1970
- NEW OBSERVATIONS ON MICROBODIESThe Journal of cell biology, 1970
- Microbodies and related particles. Morphology, biochemistry, and physiology.1969
- ENDOPLASMIC RETICULUM AND ORIGIN OF MICROBODIES IN FETAL MOUSE LIVER1967
- Hepatocellular ultrastructure during liver regeneration after subtotal hepatectomyExperimental and Molecular Pathology, 1966