Reaction of N-n-Butyl-N-nitrosourea with DNA in vitro
- 1 October 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 1 (10), 849-854
- https://doi.org/10.1093/carcin/1.10.849
Abstract
N-n-[1-14C]Butyl-N-nitrosourea was synthesized from n-[1-14C]butanol and incubated at physiological conditions (pH 7.2; 37°C) with calf thymus DNA in vitro. Following mild acid hydrolysis, purine bases were separated by Sephadex G-10 and cation exchange chromatography. 3-n-Butyladenine, 7-n-butylguanine and O6-n-butylguanine were identified as major reaction products. The O6-/7-butylguanine ratio was 0.69, indicating that the relative extent of oxygen alkylation by n-butylnitrosourea is similar to that produced by ethylnitrosourea.Keywords
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