Studies on the attachment of herpes simplex virus. Effect of trypsin treatment

Abstract

Summary The adsorption of herpes simplex virus (HSV) to monolayers of trypsinized and mock-treated GMK-AH1 cells was studied by means of a microplaque technique. Plaque formation was nearly abolished for c:a 20 minutes and full sensitivity regained at 6–8 hours post trypsinization. The return of sensitivity to HSV was inhibited by cycloheximide and incubation at 4°C. The sum of PFU in the supernatant and PFU successfully adsorbed was found to be approximately constant during the whole adsorption period. This and other experiments proved that infectivity was not destroyed during the experiments. A variety of other cell-surface modifying agents were without effect on the adsorption of HSV.