Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.

Abstract

The conditions necessary for high-level expression of methionyl bovine growth hormone (Met-bGH) in E. coli were investigated. Plasmids were constructed that contain a thermoinducible runaway replicon and either the E. coli Try or lipoportein promoter and ribosome binding sites, which served as transcriptional and trnslational initiation sites for the expression of the bGH gene. The expression of Met-bGH was low with either system. Expression levels of up to 30% of total cell protein were obtained after the introduction of additional codons 3'' to the initiating AUG codon, thus altering the NH2-terminal amino acid sequence of bGH. To obtain high-level expression of Met-bGH a 2-cistron system was constructed in which the codons that enhanced the expression of bGH were incorporated into the 1st cistron, and the coding region for Met-bGh was incorporated into the 2nd cistron. This approach may be generally applicable to achieving high-level expression of a gene that contains NH2-terminal sequences that do not allow for its efficient expression. Analyses of the stabilities of the bGH derivatives and their transcripts in vivo suggested that the variations in the level of expression were due to variations in the efficiency of mRNA translation.