Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues.
Open Access
- 1 September 1990
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 172 (3), 911-919
- https://doi.org/10.1084/jem.172.3.911
Abstract
A cDNA clone of murine macrophage inflammatory protein 2 (MIP-2) has been isolated from a library prepared from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and the nucleotide sequence determined. This cDNA was used to clone cDNAs for human homologues of MIP-2 from a library prepared from phorbol myristate acetate-treated and LPS-stimulated U937 cells. Two homologues were isolated and sequenced. Human MIP-2.alpha. and MIP-2.beta. are highly homologous to each other and to a previously isolated gene, human gro/melanoma growth-stimulating activity (MGSA). These three human genes, MIP-2.alpha., MIP-2.beta., and gro/MGSA, constitute a sub-family within the cytokine family represented by platelet factor 4 and interleukin 8.Keywords
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