Determination of extracellular space and intracellular electrolytes in rat liver in vivo*
- 1 January 1971
- journal article
- Published by Wiley in The Journal of Physiology
- Vol. 212 (1), 85-99
- https://doi.org/10.1113/jphysiol.1971.sp009311
Abstract
1. Uptake of a number of isotopically labelled extracellular tracers by rat liver in vivo was studied.2. A residual intravascular space of 3.0-3.5% following exsanguination was measured by use of [(51)Cr]red cells. This space was significantly greater than that of skeletal muscle and was also greater in nephrectomized as compared to intact rats.3. [(131)I]albumin and polyvinylpyrolidine (PVP) were found to distribute initially in a space of 6-7% and this was followed by a delayed rise that was greater for PVP. The extravascular portion of this space is termed the large-molecule accessible space and is believed to correspond to the histological space of Disse.4. [(14)C]sucrose and inulin initially distribute in a space of 10-12% followed by a continued late increase. This initial space is termed the small molecule accessible space and is thought to include most of interstitial space.5. (36)Cl(-) and stable Na(+) and Cl(-) spaces were constant at 19-21% and can be used to put an upper limit on extracellular space.6. Operationally, the 1 hr inulin space in the nephrectomized rat was felt to be the best indicator of extracellular space.7. Total electrolytes were measured and intracellular values of 22, 165 and 22 m-mole/l. cell water for Na(+), K(+) and Cl(-), respectively, were calculated.8. By use of [(14)C]DMO, an intracellular pH of 7.25 and an intracellular HCO(3) (-) of 13 m-mole/l. cell water were calculated.Keywords
This publication has 30 references indexed in Scilit:
- Glucose, Lactate and Potassium Metabolism in the Isolated Perfused Rat Liver.Experimental Biology and Medicine, 1966
- Radioautographic Studies of Inulin, Sulfate and Chloride in Rat and Guinea Pig Thyroid GlandsEndocrinology, 1965
- Extrarenale Wirkungen des Aldosterons und der SpirolactoneNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1964
- Kinetics of movement of iodide, sucrose, inulin and radio‐iodinated serum albumin in the central nervous system and cerebrospinal fluid of the ratThe Journal of Physiology, 1963
- Measurement of capacity of biliary tree in ratsAmerican Journal of Physiology-Legacy Content, 1963
- On the determination of the extracellular water compartment in swollen slices of rat liverBiochimica et Biophysica Acta, 1962
- Biliary excretion of inulin, sucrose, and mannitol: analysis of bile formationAmerican Journal of Physiology-Legacy Content, 1961
- CALCULATION OF INTRACELLULAR pH FROM THE DISTRIBUTION OF 5,5-DIMETHYL-2,4-OXAZOLIDINEDIONE (DMO). APPLICATION TO SKELETAL MUSCLE OF THE DOG*JCI Insight, 1959
- WATER DISTRIBUTION IN INCUBATED SLICES OF BRAIN AND OTHER TISSUESCanadian Journal of Biochemistry and Physiology, 1956
- On the mechanism of fluid exchange of tissues in vitroBiochemical Journal, 1956