A Staining Procedure for Use in the Brucella Opsonocytophagic Test
- 1 January 1942
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 17 (4), 153-155
- https://doi.org/10.3109/10520294209105778
Abstract
The following staining procedure is recommended for use in the Brucella opsonocytophagic test in order to avoid confusing results obtained with stains of the Hasting or Wright type: Fix spreads for 5 min. or longer in absolute methyl alcohol. Stain for 10-30 min. in a soln. of the following: 0.5 g. NaCl, 0.5 g. phenol, 0.5 g. methylene blue, 0.02 g. Na2HPO4 + 12 H2O, 50 ml. distilled water, 50 ml. methyl alcohol. Wash slides gently in water. Air dry. By this procedure, the bacteria and the nuclei of the leucocytes appear deep blue. The cytoplasm of the leucocytes appears faintly green with the cell outline distinctly visible. Cyto-plasmic granules do not stain.This publication has 1 reference indexed in Scilit:
- A Study of the Opsono-Cytophagic Power of the Blood and Allergic Skin Reaction in Brucella Infection and Immunity in ManAmerican Journal of Public Health and the Nations Health, 1933