Litcrosomal Nadph Oxidase and Hybrid Electrodes
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 15 (8), 681-698
- https://doi.org/10.1080/00032718208069502
Abstract
The NADPH oxidizing activity of rat liver microsomes was investigated and found to be mainly due to the cytochrome P-450 system. The XADPH oxidase was utilized for the development of several organelle electrodes. Gelatin membrane immobilized microsomes were combined with an O2 membrane sensor for bioelectrochemical measurement of NADPH. The dependence of the current on substrate concentration was linear up to 1 mmol·1−1 To assemble hybrid electrodes for determination of glucose-6-phoaphate, ATP and isocitrate pure enzymes were coimmobilized with the microsomal fraction.Keywords
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