Tumour-necrosis factor from the rabbit. IV. Purification and chemical characterization
Open Access
- 1 September 1980
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 42 (3), 416-422
- https://doi.org/10.1038/bjc.1980.253
Abstract
Serum from rabbits with BCG/endotoxin-induced shock is growth inhibitory or cytotoxic to a range ot tumour cell lines. The active component, tumour-necrosis factor (TNF), has been purified 1000-fold by sequential salt precipitation, ion-exchange chromatography and gel-filtration. TNF had a mol. wt of 67,000 on gradient PAGE and 39,000 on Ultrogel AcA44 gel-filtration. The isoelectric point was pH 5.1-5.2. TNF was susceptible to the proteolytic enzyme pronase, but resistant to trypsin or papain. On isopycnic ultracentrifugation it had a buoyant density of 1.27, confirming that it is protein in nature, with little or no carbohydrate. This is also suggested by its failure to bind to a range of lectins.Keywords
This publication has 8 references indexed in Scilit:
- Tumour-necrosis factor from the rabbit. III. Relationship to interferonsBritish Journal of Cancer, 1979
- Tumour-necrosis factor from the rabbit. I. Mode of action, specificity and physicochemical propertiesBritish Journal of Cancer, 1978
- [41] Peanut (Arachis hypogaea) agglutininMethods in Enzymology, 1978
- Purification of lectins by biospecific affinity chromatographyBiochimica et Biophysica Acta (BBA) - Protein Structure, 1976
- An endotoxin-induced serum factor that causes necrosis of tumors.Proceedings of the National Academy of Sciences, 1975
- The interaction of Ricinus communis agglutinin with normal and tumor cell surfacesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1972
- Polyacrylamide gel electrophoresis in a continuous molecular sieve gradientAnalytical Biochemistry, 1968
- Protein-carbohydrate interactionBiochimica et Biophysica Acta (BBA) - Protein Structure, 1967