THE ADSORPTION OF METHYL ORANGE BY LYSOZYME
- 1 March 1954
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 32 (2), 109-118
- https://doi.org/10.1139/o54-012
Abstract
Adsorption isotherms for methyl orange on lysozyme at ionic strengths varying from 0.001 to 0.05, pH 5.5, are sigmoid. Increasing ionic strength shifts the inflection point of such isotherms to higher free anion concns. Binding of one methyl orange anion to a lysozyme molecule in 0.05 [image] acetate, pH 5.5, facilitates adsorption of 9 others, with subsequent precipitation of the protein. This co-operative behavior is interpreted on the basis of a previously descr. theory of interacting hydration effects. The possible biological implications for similar systems are indicated.Keywords
This publication has 5 references indexed in Scilit:
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- THE SIZE AND SHAPE OF LYSOZYMECanadian Journal of Chemistry, 1952
- Heterogeneity of the Binding Sites of Bovine Serum Albumin1Journal of the American Chemical Society, 1950
- The Molecular Weight of Lysozyme Determined by the X-Ray Diffraction MethodJournal of the American Chemical Society, 1948
- COMPLEXES OF LYSOZYME1948