PREPARATION OF COMPLEMENT FIXING ANTIGEN FROM CRYPTOCOCCUS NEOFORMANS: PRELIMINARY REPORT

Abstract

Complement fixing antigen from Cryptococcus neoformans strains was prepared by treating the organisms with pyridine for two hours at room temperature, washing three times with buffered 0.85% sodium chloride solution, resuspending, and subsequently exposing the suspension to ultrasonic vibration for 10 min. The antigen so prepared is not anticomplementary and appears to be specific and sensitive.