Connective tissue activating peptide III induction of synthesis and secretion of plasminogen activator by synovial fibroblasts

Abstract

Connective tissue activating peptide III (CTAP-III) is a platelet factor that induces, in cultured connective tissue cells, activities observed in chronic inflammation. In this study we measured plasminogen activator secretion by synovial fibroblasts after stimulation by CTAP-III. Increased plasminogen activator secretion was observed 24—48 hours after stimulation. Induction was prevented by dexamethasone (10^-9—10^-7M), cycloheximide (1 μg/ml) and, variably, by actinomycin D (0.3 μg/ml), but not by cytosine arabinoside (10^-4M). This is the first evidence that CTAP-III induces degradative as well as proliferative activity by connective tissue cells.