Glutamate, β-Hydroxybutyrate and Succinate Dehydrogenases in Post Mortem Inner Ear Fluids

Abstract

Polyacrylamide and agarose gel electro-phoresis was used to study glutamate (GluDH), β-hy-droxybutyrate (HBDH) and succinate dehydrogenase (SDH) activity in normal human serum (NHS), normal cerebrospinal fluid (CSF) and perilymph from two cases of Menière's disease, and in corresponding post-mortem fluids, brain tissue and liver homo-genates. In post-mortem serum and in liver homo-genate, one strong band of GluDH was found at the site between the mobility of LDH₂ and LDH₃ and this band did not appear in CSF or inner ear fluids. The slower bands present in serum were absent from postmortem CSF and cochlear fluids. Activity corresponding to the sites of LDH_{1_3} was found in all fluids and control stainings. This was attributed to nonspecific reduction of NAD by the electrophoretic media.