Xanthine Oxidases in Different Species.
- 1 February 1951
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 76 (2), 252-254
- https://doi.org/10.3181/00379727-76-18452
Abstract
The enzyme present in bird tissues was a dehydrogenase rather than an oxidase, since the aerobic activity of the enzyme was negligible in comparison with its activity in the presence of methylene blue. The enzyme present in mammalian tissues was an oxidase the aerobic activity of which could generally be increased from 1.5 to 2.5 times by the addition of methylene blue to the aerobic test system. In all cases the aerobic activity of the xanthine oxidase was inhibited 40-100% by antabuse, and this inhibition was completely overcome by the addition of methylene blue. Heating the tissue homogenate at 56[degree] for 30 min. gave decreased aerobic xanthine oxidase activities with rat and mouse liver but no change with most mammalian tissues; the antabuse inhibition was eliminated to varying degrees with different tissues. The dehydrogenase enzyme or the dehydrogenase portion of the mammalian xanthine oxidase complex was unaffected by antabuse or by the heating procedure, since the activity in the presence of methylene blue remained constant irrespective of what happened to the aerobic activity.Keywords
This publication has 3 references indexed in Scilit:
- THE COMPOSITION OF RAT LIVER XANTHINE OXIDASE AND ITS INHIBITION BY ANTABUSEJournal of Biological Chemistry, 1950
- ON THE DETERMINATION OF LIVER XANTHINE OXIDASE AND THE RESPIRATION OF RAT LIVER HOMOGENATESJournal of Biological Chemistry, 1949
- The Distribution of Xanthine Oxidase IBiochemical Journal, 1926