DIGESTIVE PROCESSES OF HAEMATOPHAGOUS INSECTS: V. INHIBITORS OF TRYPSIN FROM GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE)
- 1 January 1974
- journal article
- Published by Cambridge University Press (CUP) in The Canadian Entomologist
- Vol. 106 (1), 39-44
- https://doi.org/10.4039/ent10639-1
Abstract
Trypsin has been partially purified from the midguts of Glossina morsitans. It is inactivated by tosyl-l-lysine chloromethyl ketone, phenylmethane sulfonylfluoride, and 2-mercaptoethanol. Mammalian sera inhibit G. morsitans trypsin in vitro. Sheep serum contains at least two inhibitors of G. morsitans trypsin and a high molecular weight material which protects against serum inhibition. Very low levels of trypsin inhibitor are found in G. morsitans primary excreta and in the salivary glands, Malpighian tubules, and anterior midguts of unfed flies. High concentrations of trypsin inhibitor are found in the anterior (non-digestive) section of the midgut for at least 48 h after flies fed on goats.Keywords
This publication has 6 references indexed in Scilit:
- A mosquito-chymotrypsin inhibitor in tissues of adult Aedes aegyptiComparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1972
- The interactions of Aedes aegypti (L.) trypsin with its two inhibitors found in bovine serumInsect Biochemistry, 1971
- Vertebrate serum inhibitors of Aedes aegypti trypsinInsect Biochemistry, 1971
- Factors affecting Blood Clotting from Salivary Glands and Crop of Glossina austeniNature, 1966
- The control of digestion in the tsetse fly, Glossina morsitans. Enzyme activity in relation to the size and nature of the mealJournal of Insect Physiology, 1966
- [73] Spectrophotometric and turbidimetric methods for measuring proteinsMethods in Enzymology, 1957