Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.
- 1 November 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (22), 7896-7900
- https://doi.org/10.1073/pnas.84.22.7896
Abstract
Oligodeoxynucleotides lead to translation arrest of complementary mRNAs in the wheat germ translation system by a degradation of the mRNA. In an attempt to develop an effective reverse genetic approach in vivo, we demonstrate that injection of short (15- to 30-nucleotide) oligonucleotides into Xenopus oocytes leads to complete degradation of both injected and endogenous mRNAs by means of an RNase H-like activity.Keywords
This publication has 26 references indexed in Scilit:
- Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activityCell, 1987
- The use of single-stranded DNA and RNase H to promote quantitative ‘hybrid arrest of translation’ of mRNA/DNA hybrids in reticulocyte lysate cell-free translationsNucleic Acids Research, 1986
- Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.Proceedings of the National Academy of Sciences, 1986
- Isolation and nucleotide sequence of the rabbit globin gene cluster psi zeta-alpha 1-psi alpha. Absence of a pair of alpha-globin genes evolving in concert.Journal of Biological Chemistry, 1986
- Stable reduction of thymidine kinase activity in cells expressing high levels of anti-sense RNACell, 1985
- Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryosNature, 1985
- Transcription unit of the rabbit beta 1 globin gene.Molecular and Cellular Biology, 1985
- A simple and very efficient method for generating cDNA librariesGene, 1983
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977