The Biosynthesis of the Mitochondrial ADP, ATP Translocator

Abstract
The biosynthesis of the ADP,ATP carrier was studied in mitochondria of Neurospora crassa. The carrier was isolated as the carboxyatractylate-protein complex and characterized in dodecylsulfate/polyacrylamide gel electrophoresis to have a Mr [MW] = 33,000. Applying the inhibitors chloramphenicol for the intramitochondrial translation and cycloheximide for extramitochondrial translation, the site of synthesis of this polypeptide was found to be extramitochondrially located.