Effect of bethanechol, gastrin I, or cholecystokinin on myoelectrical activity.
- 1 April 1979
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Endocrinology and Metabolism
- Vol. 236 (4), E458-E463
- https://doi.org/10.1152/ajpendo.1979.236.4.e458
Abstract
The purpose of this study was to determine the effect of bethanechol, gastrin I, or the octapeptide of cholecystokinin (CCK-OP) on the smooth muscle of the isolated cat colon. Myoelectrical activity was recorded with monopolar glass-pore electrodes. Slow-wave frequency was 5.9 +/- 0.2 cycles/min during the basal period. Slow waves were generally coupled during the control period and the apparent propagation velocity was predominantly aborad at a velocity of 3.8 +/- 0.4 mm/s. Spike activity was superimposed on 11.9 +/- 1.5% of the slow waves during the control period. Bethanechol stimulated a dose-dependent increase in colonic spike activity, with a threshold concentration of 10(-7) M. Bethanechol did not alter the congruence of the colonic slow-wave frequency at any concentration. Gastrin I or CCK-OP increased colonic spike activity. The threshold concentrations for gastrin I and CCK-OP were 2 X 10(-11) M and 4 x 10(-11) M, respectively. Unlike bethanechol, gastrin I (2 X 10(-9) M - 2 X 10(-8) M) and CCK-OP (4 X 10(-9) - 4 X 10(-8) M) altered slow-wave frequency and decreased slow-wave congruence. These studies suggest that 1) bethanechol, gastrin I, or CCK-OP increases colonic spike activity, and 2) only gastrin I or CCK-OP alters the slow-wave frequency of colonic muscle. Thus neurohumoral substances may act independently on colonic spike activity and colonic slow-wave frequency.This publication has 27 references indexed in Scilit:
- The Generation and Conduction of Activity in Smooth MuscleAnnual Review of Pharmacology and Toxicology, 1978
- Interaction of gastrin I, secretin, and cholecystokinin on gallbladder smooth muscleAmerican Journal of Physiology-Legacy Content, 1976
- The effects of graded doses of C-terminal octapeptide of cholecystokinin on small intestinal transit time in manDigestive Diseases and Sciences, 1974
- Simulation of the electric-control activity of the stomach by an array of relaxation oscillatorsDigestive Diseases and Sciences, 1972
- SARCOPLASMIC RETICULUM AND EXCITATION-CONTRACTION COUPLING IN MAMMALIAN SMOOTH MUSCLESThe Journal of cell biology, 1972
- Excitation-contraction coupling and electrical events in two types of vascular smooth muscleMicrovascular Research, 1969
- Electric slow waves in circular muscle of cat colonAmerican Journal of Physiology-Legacy Content, 1969
- Simulation of the electrical and mechanical gradient of the small intestineAmerican Journal of Physiology-Legacy Content, 1968
- Comparison of the effects of a gastrin extract and a synthetic pentapeptide on gastrointestinal motility in the cat.Gut, 1967
- THE EFFECT OF A SYNTHETIC GASTRIN-LIKE PENTAPEPTIDE (I.C.I. 50,123) ON INTESTINAL MOTILITY IN MANThe Lancet, 1966