Abstract
1. Arteriovenous differences of amino acids across the lactating mammary gland were measured in normal rats and weaned for 4, 5 and 24h. 2. Uptake of amino acids by mammary glands of rats weaned for 5h or more was significantly lower than that of controls. This was not reversed by injection of prolactin. 3. By using ‘unilaterally weaned’ rats we showed that milk accumulation plays an important role in amino acid uptake by mammary gland. 4. gamma-Glutamyltransferase activity was significantly lower in ‘weaned’ glands than in ‘normal’ glands. This provides further support for the hypothesis of the function of the gamma-glutamyl cycle in the mammary gland in vivo.