Glutathione-Dependent Dehydroascorbate Reduction: a Determinant of Dehydroascorbate Uptake by Human Polymorphonuclear Leukocytes

Abstract

Dehydroascorbate uptake and reduction are impaired in normal human polymorphonuclear leukocytes (PMN) preincubated with N-ethylmaleimide, and in glutathione reductase-deficient cells. We conclude that human PMN dehydroascorbate reduction is glutathione-dependent, and that dehydroascorbate reduction appears to be a necessary condition for cellular dehydroascorbate uptake.