Mapping of the genes coding for the two major vaccinia virus core polypeptides
Open Access
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (12), 4835-4848
- https://doi.org/10.1093/nar/12.12.4835
Abstract
We have mapped the genes coding for two major structural polypeptides of the vaccinia virus core by hybrid selection and transcriptional mapping. First, RNA was selected by hybridization to restriction fragments of the vaccinia virus genome, translated in vitro and the products were immunoprecipitated with antibodies against the two polypeptides. This approach allowed us to map the genes to the left hand end of the largest Hind III restriction fragment of 50 kilobase pairs. Second, transcriptional mapping of this region of the genome revealed the presence of the two expected RNAs. Both RNAs are transcribed from the leftward reading strand and the 5′-ends of the genes are separated by about 7.5 kilobase pairs of DNA. Thus, two genes encoding structural polypeptides with a similar location in the vaccinia virus particle are clustered at approximately 105 kilobase pairs from the left hand end of the 180 kilobase pair vaccinia virus genome.Keywords
This publication has 14 references indexed in Scilit:
- Construction of live vaccines using genetically engineered poxviruses: biological activity of vaccinia virus recombinants expressing the hepatitis B virus surface antigen and the herpes simplex virus glycoprotein D.Proceedings of the National Academy of Sciences, 1984
- Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutationsJournal of Virology, 1983
- Infectious vaccinia virus recombinants that express hepatitis B virus surface antigenNature, 1983
- Vaccinia virus: a selectable eukaryotic cloning and expression vector.Proceedings of the National Academy of Sciences, 1982
- Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell-free translation of selected mRNAProceedings of the National Academy of Sciences, 1982
- Distinctive nucleotide sequences adjacent to multiple initiation and termination sites of an early vaccinia virus geneCell, 1981
- Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15 S β-globin mRNA precursor and mature 10 S β-globin mRNA have identical map coordinatesNucleic Acids Research, 1979
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Vaccinia Virus Morphogenesis: A Comparison of Virus-Induced Antigens and PolypeptidesJournal of General Virology, 1973
- RNA polymerase activity in purified infectious vaccinia virus.Proceedings of the National Academy of Sciences, 1967