The dehydrogenases of Bact. coli

Abstract

Dilution of suspensions of Bact. coli results in a considerable falling off in activity of lactic acid dehydrogenase, which has been shown to be due to the presence of a heat-stable coenzyme. The coenzyme is replaceable by the cozymase of yeast fermentation. Similar results were obtained with the cell-free preparation of lactic dehydrogenase of Stephenson. These findings recall the results previously obtained with the glucose dehydrogenase of Bact. coli. The affinities for lactate of the lactic dehydrogenase of intact cells and the soluble preparation show slight but constant differences.